BPC-157

BPC 157 is a penta-decapeptide composed of 15 amino acids. It is a partial sequence of the body protection compound (BPC) that was isolated from gastric juice. Animal studies show accelerated healing of many different wounds, including muscle, tendon and damaged ligaments. Additionally, BPC 157 has shown to protect organs and aids in the prevention…

Description

BPC-157 (Body Protection Compound-157) is an injectable pentadecapeptide made up of 15 amino acids. The amino acids sequence in BPC-157 is similar to a portion of the human BPC amino acid sequence. Human BPC is found in the gastric juice. Experiments have shown that BPC-157 enhances the healing of wounds, including tendons wounds such as transected Achilles tendons of rats. The aim of this study was to investigate the probable mechanism that BPC-157 utilizes to accelerate the healing process in an injured tendon. The study used two group of tendon explants of which one group was cultured in a BPC-157 containing medium while the other group was cultured in a medium lacking BPC-157. These cultures were thereafter examined for tendon fibroblasts outgrowths. Such outgrowths indicated tendon regeneration.The results revealed that the explants’ outgrowth was significantly accelerated in the culture containing BPC-157 as compared to the culture lacking BPC-157. Also, a MTT assay did show that BPC-157 does not directly affect cellular proliferation in a culture of rat-derived Achilles tendon. However, results also showed that BPC-157 significantly increased the survival of cells under oxidative stress. Furthermore, the Transwell filter migration assay showed that BPC-157 significantly increased in-vitro fibroblast migration in a dose-dependent fashion. Moreover, BPC-157 accelerated the dispersal of the fibroblasts in culture dishes in a dose-dependent manner.

Additionally, FITC-phalloidin staining was able to demonstrate that BPC-157 induces F-actin formation in fibroblasts. Likewise, Western blot analysis was able to detect the production and activation of paxillin and FAK proteins. The western blot analysis also showed that BPC-157 increases the extent of phosphorylation of paxillin and FAK proteins without affecting the amounts produced.

Thus, it can be concluded that BPC-157 enhances the ex-vivo growth and in-vitro cellular migration of fibroblasts derived from rat tendon explants. Moreover, BPC-157 also increases the probability of a cell surviving under oxidative stress. These actions of BPC-157 are probably mediated by the activation (through phosphoryl) of the proteinic FAK-paxillin pathway.